S2Media™ Mueller Hinton II Broth, Cation Adjusted, is a non-selective, non-differential, and non-enriched microbiological growth medium primarily used for antimicrobial susceptibility testing, particularly with the broth microdilution method. The cation adjustment (optimized levels of calcium and magnesium) ensures reproducibility and accuracy in susceptibility testing, especially for aminoglycosides and tetracyclines. It supports the growth of a wide range of non-fastidious organisms.• Product meets ASM, CLSI and WHO reference methods and performance criteria.• Mueller Hinton II Broth should be used by trained professionals within a laboratory setting. • Product is not intended for use in the diagnosis or treatment of disease or other human conditions.